BioResearch BioResearch

1. Protein and nucleotide nanolayers for biosensor applications
1.1. Microscopic (AFM, AFM-TREC, SNOM, fluorescence) and spectroscopic (TOF-SIMS, ARXPS) analysis of biorecognition (protein and nucleotide) nanolayers for biosensor applications [cooperation: P. Petrou, Athens]
1.2. Protein surface micro-patterning with self-assembly and soft lithography (AFM, NSOM, fluorescence, TOF-SIMS, combinatorial surface chemistry) [cooperation M. Lekka, Kraków]
2. Effects of man-made organic surfaces on protein and cell behavior
2.1. Intelligent (stimuli-responsive) and biomimetic organic coatings for controlled protein adsorption (AFM, fluorescence, TOF-SIMS, contact angle) [cooperation Y. Stetsyshyn, Lvov]
2.2. Effects of artificial micro- and nano-structured polymer surfaces on cell behavior (AFM, optical, fluorescence, TOF-SIMS, contact angle) [cooperation M. Lekka, Kraków]



"Electron-Beam Lithographic Grafting of Functional Polymer Structures from Fluoropolymer Substrates" K. Gajos, V. A. Guzenko, M. Duebner, J. Haberko, A. Budkowski, C. Padeste, Langmuir 32 (2016), 10641–10650

"Indirect immunoassay on functionalized silicon surface: Molecular arrangement, composition and orientation examined step-by-step with multi-technique and multivariate analysis” K. Gajos, A. Budkowski, V. Pagkali, P. Petrou, M. Biernat, K. Awsiuk, J. Rysz, A. Bernasik, K. Misiakos, I. Raptis, S. Kakabakos, Colloids and Surfaces B: Biointerfaces (2016) in press

"Comparing surface properties of melanoma cells using time of flight secondary ions mass spectrometry" J. Bobrowska, J. Moffat, K. Awsiuk, J. Pabijan, J. Rysz, A. Budkowski, M. Reading, M. Lekka, Analyst 141 (2016), 6217-6225

"The cholesterol-based grafted polymer brushes as alignment coating with temperature-tuned anchoring for nematic liquid crystals" Y. Stetsyshyn, J. Raczkowska, A. Budkowski, K. Awsiuk, A. Kostruba, S. Nastyshyn, K. Harhay, E. Lychkovskyy, H. Ohar, Y. Nastishin, Langmuir 32 (2016), 11029−11038 

"Effect of substrate elasticity on macroscopic parameters of fish keratocyte migration" D. Dziob, T. Kołodziej, J. Nowak, P. Cyzio, J. Raczkowska, J. Laska, Z. Rajfur, Physical Biology 13 (2016) 054001

"Precise positioning of cancerous cells on PDMS substrates with gradients of elasticity" J. Raczkowska, S. Prauzner-Bechcicki, Biomedical Microdevices 18 (2016), 1-8

"Orientation and biorecognition of immunoglobulin adsorbed on spin-cast poly(3-alkylthiophenes): impact of polymer film crystallinity" K. Awsiuk, A. Budkowski, P. Petrou, M. M. Marzec, M. Biernat, T. Jaworska-Gołąb, J. Rysz, Colloids and Surfaces B: Biointerfaces 148 (2016), 278-286

"Protocol of single cells preparation for time of flight secondary ion mass spectrometry" J. Bobrowska, J. Pabijan, J. Wiltowska-Zuber, B.R. Jany, F. Krok, K. Awsiuk, J. Rysz, A. Budkowski, M. Lekka, Analytical Biochemistry 511 (2016), 52-60

"Data on step-by-step atomic force microscopy monitoring of changes occurring in single melanoma cells undergoing ToF SIMS specialized sample preparation protocol" J. Bobrowska, J. Pabijan, J. Wiltowska-Zuber, B.R. Jany, F. Krok, K. Awsiuk, J. Rysz, A. Budkowski, M. Lekka, Data in Brief 8 (2016), 1322-1332

"Physico-chemical properties of PDMS surfaces suitable as substrates for cell cultures" J. Raczkowska, S. Prauzner-Bechcicki, J. Lukes, J. Sepitka, A. Bernasik, K. Awsiuk, C. Paluszkiewicz, J. Pabijan, M. Lekka, A. Budkowski, Applied Surface Science 389 (2016), 247–254

"Imaging and chemical surface analysis of biomolecular functionalization of monolithically integrated on silicon Mach-Zehnder interferometric immunosensors" K. Gajos, M. Angelopoulou, P. Petrou, K. Awsiuk, S. Kakabakos, W. Haasnoot, A. Bernasik, J. Rysz, M. M. Marzec, K. Misiakos, I. Raptis, A. Budkowski, Applied Surface Science 385 (2016), 529-542

"Synthesis and Postpolymerization Modification of Thermoresponsive Coatings Based on Pentaerythritol Monomethacrylate: Surface Analysis, Wettability, and Protein Adsorption" Y. Stetsyshyn, J. Raczkowska, A. Budkowski, A. Kostruba, K. Harhay, H. Ohar, K. Awsiuk, A. Bernasik, N. Ripak, J. Zemła, Langmuir 31 (2015), 9675–9683

"Direct Covalent Biomolecule Immobilization on Plasma-Nanotextured Chemically Stable Substrates" K. Tsougeni, P. S. Petrou, K. Awsiuk, M. M. Marzec, N. Ioannidis, V. Petrouleas, A. Tserepi, S. E. Kakabakos, E. Gogolides, ACS Applied Materials and Interfaces 7 (2015), 14670–14681

"Improved DNA microarray detection sensitivity through immobilization of preformed in solution streptavidin/biotinylated oligonucleotide conjugates" E. Mavrogiannopoulou, G. Koukouvinos, D. Yannoukakos, A. Siafaka-Kapadai, K. Fornal, K. Awsiuk, A. Budkowski, S.E. Kakabakos, Colloids and Surfaces B: Biointerfaces 128 (2015), 464–472

"Differentiation between Single Bladder Cancer Cells Using Principal Component Analysis of Time-of-Flight Secondary Ion Mass Spectrometry" J. Gostek, K. Awsiuk, J. Pabijan, J. Rysz, A. Budkowski, M. Lekka, Analytical Chemistry 87 (2015), 3195–3201

"Imaging and spectroscopic comparison of multi-step methods to form DNA arrays based on the biotin-streptavidin system" K. Gajos, P. Petrou, A. Budkowski, K. Awsiuk, A. Bernasik, K. Misiakos, J. Rysz, I. Raptis, S. Kakabakos, Analyst 140 (2015), 1127–1139

"PDMS substrate stiffness affects the morphology and growth profiles of cancerous prostate and melanoma cells" S. Prauzner-Bechcicki, J. Raczkowska, E. Madej, J. Pabijan, J. Lukes, J. Sepitka, J. Rysz, K. Awsiuk, A. Bernasik, A. Budkowski, M. Lekka, Journal of the Mechanical Behavior of Biomedical Materials 41 (2015), 13–22

"Effects of polythiophene surface structure on adsorption and conformation of bovine serum albumin: A multivariate and multitechnique study" K. Awsiuk, A. Budkowski, M.M. Marzec, P. Petrou, J. Rysz, A. Bernasik, Langmuir 30 (2014), 13925–13933

"Temperature-responsive peptide-mimetic coating based on poly(N-methacryloyl-L-leucine): Properties, protein adsorption and cell growth" J. Raczkowska, M. Ohar, Y. Stetsyshyn, J. Zemła, K. Awsiuk, J. Rysz, K. Fornal, A. Bernasik, H. Ohar, S. Fedorova, O. Shtapenko, S. Polovkovych, V. Novikov, A. Budkowski, Colloids and Surfaces B: Biointerfaces 118 (2014), 270–279

"Immobilization of oligonucleotide probes on silicon surfaces using biotin–streptavidin system examined with microscopic and spectroscopic techniques" K. Awsiuk, J. Rysz, P. Petrou, A. Budkowski, A. Bernasik, S. Kakabakos, M.M. Marzec, I. Raptis, Applied Surface Science 290 (2014) 199–206

"Plasma-Assisted Nanoscale Protein Patterning on Si Substrates via Colloidal Lithography" A. Malainou, K. Tsougeni, K. Ellinas, P. S. Petrou, V. Constantoudis, E. Sarantopoulou, K. Awsiuk, A. Bernasik ,A. Budkowski ,A. Markou ,I. Panagiotopoulos ,S. E. Kakabakos, E. Gogolides, and A. Tserepi The Journal of Physical Chemistry A 117 (2013), 13743–13751

"Temperature and pH dual-responsive POEGMA-based coatings for protein adsorption" Y. Stetsyshyn,K. Fornal, J. Raczkowska, J. Zemla, A. Kostruba, Halyna Ohar, Mariya Ohar, Volodymyr Donchak, Khrystyna Harhay,K. Awsiuk,J. Rysz, A. Bernasik, A. Budkowski, Journal of Colloid and Interface Science 411 (2013) 247–256

"Protein adsorption and covalent bonding to silicon nitride surfaces modified with organo-silanes: comparison using AFM, angle-resolved XPS and multivariate ToF-SIMS analysis", K. Awsiuk, A. Budkowski, A. Psarouli, P. Petrou, A. Bernasik, S. Kakabakos, J. Rysz, I. Raptis, Colloids and Surfaces B: Biointerfaces 110 (2013) 217-224

"Model immunoassay on silicon surfaces: vertical and lateral nanostructure vs. protein coverage", K. Awsiuk, A. Budkowski, P. Petrou, A. Bernasik, M. M. Marzec, S. Kakabakos, J. Rysz, I. Raptis Colloids and Surfaces B: Biointerfaces 103 (2013) 253–260

"Temperature and pH Dual-Responsive Coatings of Oligoperoxide-graft-Poly(N-isopropylacrylamide): Wettability, Morphology and Protein Adsorption" Y. Stetsyshyn, J. Zemla, O. Zolobko, K. Fornal, A. Budkowski, A. Kostruba, V. Donchak, K. Harhay, K. Awsiuk, J. Rysz, A. Bernasik, S. Voronov, Journal of Colloid and Interface Science 387(2012)95–105

"Proteins grouped into a variety of regular micro-patterns by substrate-guided domains of self-assembling poly(ethylene oxide)/ polystyrene blends", J. Zemła, J. Rysz, A. Budkowski, K. Awsiuk, Soft Matter 8 (2012) 5550-5560

"Implementation of NSOM to Biological Samples", S. Prauzner-Bechcicki, J. Wiltowska-Zuber, A. Budkowski, M. Lekka and J. Rysz, Acta Physica Polonica A 121 (2012) 533-538

"Spectroscopic and microscopic characterization of biosensor surfaces with protein/amino-organosilane/silicon structure", K. Awsiuk, A. Bernasik, M. Kitsara, A. Budkowski, P. Petrou, S. Kakabakos, S. Prauzner-Bechcicki, J. Rysz, I. Raptis, Colloids and Surfaces B: Biointerfaces 90 (2012) 159-168

"Reverse contrast and substructures in protein micro-patterns on 3D polymer surfaces", J. Zemła, A. Budkowski, J. Rysz, J. Raczkowska, M. Lekka, Colloids and Surfaces B: Biointerfaces 90 (2012) 144-151

"Spectroscopic and microscopic examination of protein adsorption and blocking of non-specific binding to silicon surfaces modified with APTES and GOPS" K. Awsiuk, A. Psarouli, P. Petrou, A. Budkowski, S. Kakabakos, A. Bernasik, J. Rysz, I. Raptis, Procedia Engineering 25 (2011) 334-337

"Polymer Blends Spin-cast into Films with Complementary Elements for Electronics and Biotechnology" A. Budkowski,J. Zemła, E. Moons, K. Awsiuk, J. Rysz, A. Bernasik, C.M. Björström-Svanström, M. Lekka, J. Jaczewska, Journal of Applied Polymer Science 125 (2012) 4275–4284

"Protein coverage on silicon surfaces modified with amino-organic films: A study by AFM and angle-resolved XPS", K. Awsiuk, A. Bernasik, M. Kitsara, A. Budkowski, J. Rysz, J. Haberko, P. Petrou, K. Beltsios, J. Raczkowska, Colloids and Surfaces B: 80 (2010) 63-71

"Formation, structure and wettability of fluorescent nanolayers of oligoperoxide europium complexes adsorbed to glass surface", Y. Stetsyshyn, A. Kostruba, J. Jaczewska, A. Zaichenko, N. Mitina, A. Budkowski, P. Kuzyk, A. Voloshinovskii, Thin Solid Films 518 (2010) 4318-4321

"Selective protein adsorption on polymer patterns formed by self-organization and soft lithography", J. Zemła, M. Lekka, J. Raczkowska, A. Bernasik, J. Rysz, A. Budkowski, Biomacromolecules 10 (2009) 2101-2109

"Modification of poly(ethylene terephtalate) surface by attached dextran macromolecules" , Y. Stetsyshyn, V. Donchak, K. Harhay, S. Voronov, J. Raczkowska, A. Budkowski, Polymer International 58 (2009) 1034-1040

"Structures in multi-component polymer films: their formation, observation and applications in electronics and biotechnology" , A. Budkowski, A. Bernasik, E. Moons, M. Lekka, J. Zemła, J. Jaczewska, J. Haberko, J. Raczkowska, J. Rysz, K. Awsiuk, Acta Physica Polonica A 115 (2009) 435-440

"Integral geometry analysis of fluorescence micrographs for quantitative relative comparison of protein adsorption onto polymer surfaces" J. Zemla, M. Lekka, J. Wiltowska-Zuber, A. Budkowski, J. Rysz, J. Raczkowska, Langmuir 24 (2008) 10253-10258

"Friction force microscopy as an alternative method to probe molecular interaction", M. Lekka, A. J. Kulik, S. Jeney, J. Raczkowska, J.Lekki, A. Budkowski, L.Forró, J. Chem. Phys. 123 (2005) 014702 (-1-7)

"Binding activity of patterned concanavalin A studied by atomic force microscopy", K. Lebed, G. Pyka-Fosciak, J. Raczkowska, M. Lekka and J. Styczen, J. Phys.: Cond. Matt. 17 (2005) S1447-S1458